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Towards an integrated device that utilizes adherent cells in a micro-free-flow electrophoresis chip to achieve separation and biosensing.

Authors
  • Jezierski, Stefan
  • Klein, Anke S
  • Benz, Christian
  • Schaefer, Michael
  • Nagl, Stefan
  • Belder, Detlev
Type
Published Article
Journal
Analytical and Bioanalytical Chemistry
Publisher
Springer-Verlag
Publication Date
Jun 01, 2013
Volume
405
Issue
16
Pages
5381–5386
Identifiers
DOI: 10.1007/s00216-013-6945-7
PMID: 23591645
Source
Medline
License
Unknown

Abstract

We immobilized adherent human embryonic kidney (HEK) cells--which are able to trace adenosine triphosphate (ATP)--inside a microfluidic free-flow electrophoresis (μFFE) chip in order to develop an integrated device combining separation and biosensing capabilities. HEK 293 cells loaded with fluorescent calcium indicators were used as a model system to enable the spatially and temporally resolved detection of ATP. The local position of a 20 μM ATP stream was successfully visualized by these cells during free-flow electrophoresis, demonstrating the on-line detection capability of this technique towards native, unlabeled compounds.

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