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Toward a functional annotation of the human genome using artificial transcription factors.

Authors
  • Lee, Dong-ki
  • Park, Jin Woo
  • Kim, Youn-Jae
  • Kim, Jiwon
  • Lee, Yangsoon
  • Kim, Jeonglim
  • Kim, Jin-Soo
Type
Published Article
Journal
Genome Research
Publisher
Cold Spring Harbor Laboratory
Publication Date
Dec 01, 2003
Volume
13
Issue
12
Pages
2708–2716
Identifiers
PMID: 14656973
Source
Medline
License
Unknown

Abstract

We have developed a novel, high-throughput approach to collecting randomly perturbed gene-expression profiles from the human genome.A human 293 cell library that stably expresses randomly chosen zinc-finger transcription factors was constructed, and the expression profile of each cell line was obtained using cDNA microarray technology.Gene expression profiles from a total of 132 cell lines were collected and analyzed by (1) a simple clustering method based on expression-profile similarity, and (2) the shortest-path analysis method. These analyses identified a number of gene groups, and further investigation revealed that the genes that were grouped together had close biological relationships. The artificial transcription factor-based random genome perturbation method thus provides a novel functional genomic tool for annotation and classification of genes in the human genome and those of many other organisms.

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