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Toward Developing a Yeast Cell Factory for the Production of Prenylated Flavonoids.

Authors
  • Levisson, Mark
  • Araya-Cloutier, Carla1
  • de Bruijn, Wouter J C1
  • van der Heide, Menno
  • Salvador López, José Manuel
  • Daran, Jean-Marc2
  • Vincken, Jean-Paul1
  • Beekwilder, Jules
  • 1 Laboratory of Food Chemistry , Wageningen University & Research , Bornse Weilanden 9 , 6708 WG Wageningen , Netherlands. , (Netherlands)
  • 2 Department of Biotechnology , Delft University of Technology , Van der Maasweg 9 , 2629 HZ Delft , Netherlands. , (Netherlands)
Type
Published Article
Journal
Journal of Agricultural and Food Chemistry
Publisher
American Chemical Society
Publication Date
Dec 11, 2019
Volume
67
Issue
49
Pages
13478–13486
Identifiers
DOI: 10.1021/acs.jafc.9b01367
PMID: 31016981
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Prenylated flavonoids possess a wide variety of biological activities, including estrogenic, antioxidant, antimicrobial, and anticancer activities. Hence, they have potential applications in food products, medicines, or supplements with health-promoting activities. However, the low abundance of prenylated flavonoids in nature is limiting their exploitation. Therefore, we investigated the prospect of producing prenylated flavonoids in the yeast Saccharomyces cerevisiae. As a proof of concept, we focused on the production of the potent phytoestrogen 8-prenylnaringenin. Introduction of the flavonoid prenyltransferase SfFPT from Sophora flavescens in naringenin-producing yeast strains resulted in de novo production of 8-prenylnaringenin. We generated several strains with increased production of the intermediate precursor naringenin, which finally resulted in a production of 0.12 mg L-1 (0.35 μM) 8-prenylnaringenin under shake flask conditions. A number of bottlenecks in prenylated flavonoid production were identified and are discussed.

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