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Total flavones of Rhododendron simsii Planch flower protect rat hippocampal neuron from hypoxia-reoxygenation injury via activation of BKCa channel.

Authors
  • Guo, Yan1
  • Yu, Xiao-Meng1
  • Chen, Shuo1
  • Wen, Ji-Yue1
  • Chen, Zhi-Wu1
  • 1 Department of Pharmacology, Anhui Medical University, Hefei, China. , (China)
Type
Published Article
Journal
The Journal of pharmacy and pharmacology
Publication Date
Jan 01, 2020
Volume
72
Issue
1
Pages
111–120
Identifiers
DOI: 10.1111/jphp.13178
PMID: 31598976
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

To study the effects of total flavones of Rhododendra simsii Planch flower (TFR) on hypoxia/reoxygenation (H/R) injury in rat hippocampal neurons and its underlying mechanism. Model of H/R was established in newborn rat primary cultured hippocampal neuron. Lactate dehydrogenase (LDH) and neuron-specific enolase (NSE) activity as well as malondialdehyde (MDA) content in cultured supernatants of the neurons were examined. Methyl thiazolyl tetrazolium assay and Hoechst33258 staining were, respectively, used to detect cell viability and apoptosis of neurons. Protein expression and current of BKCa channel were assessed by using Western blotting and whole-cell patch-clamp methods, respectively. In the ranges of 3.7-300 mg/l, TFR significantly inhibited H/R-induced decrease of neuronal viability and increases of LDH, NSE and MDA in the supernatants as well as apoptosis; TFR 33.3, 100 and 300 mg/l markedly increased current of BKCa channel rather than the BKCa channel protein expression in the neurons. Total flavones of R. simsii Planch flower had a protective effect against H/R injury in rat hippocampal neuron, and activation of BKCa channel may contribute to the neuroprotection. © 2019 Royal Pharmaceutical Society.

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