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TORC1 regulates ESCRT-0 complex formation on the vacuolar membrane and microautophagy induction in yeast.

Authors
  • Morshed, Shamsul1
  • Sharmin, Tasnuva1
  • Ushimaru, Takashi2
  • 1 Graduate School of Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, 422-8021, Japan. , (Japan)
  • 2 Graduate School of Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, 422-8021, Japan; Department of Science, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, 422-8021, Japan. Electronic address: [email protected] , (Japan)
Type
Published Article
Journal
Biochemical and Biophysical Research Communications
Publisher
Elsevier
Publication Date
Jan 29, 2020
Volume
522
Issue
1
Pages
88–94
Identifiers
DOI: 10.1016/j.bbrc.2019.11.064
PMID: 31740006
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Microautophagy is promoted after nutrient starvation and inactivation of target of rapamycin complex 1 (TORC1) kinase. Invagination of vacuolar membranes by endosomal sorting complex required for transport (ESCRT) is required for microautophagy. Vps27, a subunit of ESCRT-0, is recruited onto vacuolar membranes via dephosphorylation after TORC1 inactivation. Here, we showed that Hse1, another ESCRT-0 subunit, is also recruited onto vacuolar membranes after TORC1 inactivation, promoting formation of ESCRT-0 complex on vacuolar membranes. Hse1 recruitment was dependent on Vps27, whereas Vps27 recruitment was independent of Hse1. Not only Vps27 but also Hse1 was required for ESCRT-III recruitment onto vacuolar membranes and microautophagy induction after TORC1 inactivation. This study revealed that ESCRT-0 (Vps27-Hse1) complex formation on vacuolar membranes is important for microautophagy inactivation after TORC1 inactivation. Copyright © 2019 Elsevier Inc. All rights reserved.

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