Affordable Access

deepdyve-link
Publisher Website

TORC1 inactivation stimulates autophagy of nucleoporin and nuclear pore complexes.

Authors
  • Tomioka, Yui1
  • Kotani, Tetsuya1
  • Kirisako, Hiromi1
  • Oikawa, Yu2
  • Kimura, Yayoi3
  • Hirano, Hisashi3
  • Ohsumi, Yoshinori2
  • Nakatogawa, Hitoshi1
  • 1 School of Life Science and Technology, Tokyo Institute of Technology, Yokohama, Japan. , (Japan)
  • 2 Institute of Innovative Research, Tokyo Institute of Technology, Yokohama, Japan. , (Japan)
  • 3 Advanced Medical Research Center, Yokohama City University, Yokohama, Japan. , (Japan)
Type
Published Article
Journal
The Journal of Cell Biology
Publisher
The Rockefeller University Press
Publication Date
Jul 06, 2020
Volume
219
Issue
7
Identifiers
DOI: 10.1083/jcb.201910063
PMID: 32453403
Source
Medline
Language
English
License
Unknown

Abstract

The mechanisms underlying turnover of the nuclear pore complex (NPC) and the component nucleoporins (Nups) are still poorly understood. In this study, we found that the budding yeast Saccharomyces cerevisiae triggers NPC degradation by autophagy upon the inactivation of Tor kinase complex 1. This degradation largely depends on the selective autophagy-specific factor Atg11 and the autophagy receptor-binding ability of Atg8, suggesting that the NPC is degraded via receptor-dependent selective autophagy. Immunoelectron microscopy revealed that NPCs embedded in nuclear envelope-derived double-membrane vesicles are sequestered within autophagosomes. At least two pathways are involved in NPC degradation: Atg39-dependent nucleophagy (selective autophagy of the nucleus) and a pathway involving an unknown receptor. In addition, we found the interaction between Nup159 and Atg8 via the Atg8-family interacting motif is important for degradation of this nucleoporin not assembled into the NPC. Thus, this study provides the first evidence for autophagic degradation of the NPC and Nups, which we term "NPC-phagy" and "nucleoporinophagy." © 2020 Tomioka et al.

Report this publication

Statistics

Seen <100 times