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Topology of RbsC, the Membrane Component of the Escherichia coli Ribose Transporter†

Authors
Publisher
American Society for Microbiology
Publication Date
Source
PMC
Keywords
Disciplines
  • Biology
  • Design
  • Mathematics
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Unknown

Abstract

The topology of RbsC, the membrane component of the ribose transporter in Escherichia coli, has been determined by using 34 single-cysteine mutants and a modified fluorescence labeling technique designated multiplex labeling. This technique gives topology, expression, and localization information for a membrane protein from a single batch of bacterial cells. The results indicate that RbsC contains 10 transmembrane-spanning helices, with the N and C termini being in the cytosol. This topology matches predictions from the latest prediction programs and the topology of the similar, recently crystallized membrane protein BtuC.

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