Affordable Access

Topography of basal glucose utilization in rat thalamus and hypothalamus determined with (1-14C)-glucose.

Authors
Type
Published Article
Journal
Anatomy and embryology
Publication Date
Volume
179
Issue
1
Pages
1–17
Identifiers
PMID: 3213951
Source
Medline

Abstract

High resolution autoradiography was used to study the basal pattern of glucose-utilization in the rat thalamus and hypothalamus. Rats were injected via chronic jugular catheter with (1-14C)-glucose and sacrificed 30 min later. The high resolution thaw-mount autoradiographic procedure, using 4 micron frozen sections and nuclear emulsion, permitted discrimination of regional variations in glucose-utilization that have not yet been described. Quantitative data were obtained by means of digital image analysis and computerized densitometry. In the thalamus, high activity was present in the anterodorsal, anteroventral, laterodorsal and reticular nuclei, while low activity was found in the mediodorsal and paraventricular nuclei. The autoradiographic pattern of glucose utilization in the thalamus corresponds largely to classical cytoarchitectonic subdivisions. In the hypothalamus, the median eminence, arcuate nucleus, and periventricular nucleus showed the lowest activity, whereas certain parts of the lateral hypothalamus appeared high. Very high activity was present in mammillary nuclei. The described detailed anatomical data of glucose-utilization may provide insights into the functional circuitry of thalamic and hypothalamic systems and serve as a baseline from which experimental manipulations can be assessed.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments