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Topographical Location of H—Y Antigen on Mouse Spermatozoa by Immunoelectronmicroscopy

Authors
  • Gloria C. Koo
  • Christopher W. Stackpole
  • Edward A. Boyse
  • Ulrich Hämmerling
  • Michael P. Lardis
Publication Date
May 01, 1973
Source
PMC
Keywords
License
Unknown

Abstract

H-Y (male) antigen was visually located on mouse sperm by electron microscopy, by use of the indirect hybrid antibody method and tobacco mosaic virus as the visual marker. Labeling was achieved by centrifugation of the sperm through a discontinuous gradient consisting of alternating layers of immune reagents and wash solutions. Treated sperm were examined topographically by preparation of platinum-carbon replicas. Antigen was located mainly on the acrosomal cap of the sperm head.

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