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Tn9 CAT gene contains a promoter for vaccinia virus transcription: implications for reverse-genetic techniques.

Authors
Type
Published Article
Journal
Journal of Virological Methods
0166-0934
Publisher
Elsevier
Publication Date
Volume
36
Issue
1
Pages
85–90
Identifiers
PMID: 1313041
Source
Medline
License
Unknown

Abstract

Vaccinia virus-dependent CAT expression was observed in virus-infected cells cotransfected with a promoterless CAT gene. Restriction endonuclease resection of the CAT plasmid indicated that expression was due to recognition by vaccinia virus RNA polymerase of sequences within the CAT gene itself, probably located within the 5' untranslated region of the gene. This observation is relevant to the design of reverse-genetic systems which use CAT as a reporter gene to detect replication of negative-strand RNA virus pseudogenomes.

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