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Tissue-engineered human embryonic stem cell-containing cardiac patches: evaluating recellularization of decellularized matrix.

Authors
  • Hochman-Mendez, Camila1, 2
  • Pereira de Campos, Dilza Balteiro1, 2
  • Pinto, Rafael Serafim1
  • Mendes, Bernardo Jorge da Silva1
  • Rocha, Gustavo Miranda1
  • Monnerat, Gustavo1
  • Weissmuller, Gilberto1
  • Sampaio, Luiz C2
  • Carvalho, Adriana Bastos1, 3
  • Taylor, Doris A2
  • de Carvalho, Antonio Carlos Campos1, 3
  • 1 Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. , (Brazil)
  • 2 Regenerative Medicine Research, Texas Heart Institute, Houston, TX, USA.
  • 3 National Institute of Science and Technology for Regenerative Medicine, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. , (Brazil)
Type
Published Article
Journal
Journal of tissue engineering
Publication Date
Jan 01, 2020
Volume
11
Identifiers
DOI: 10.1177/2041731420921482
PMID: 32742631
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Decellularized cardiac extracellular matrix scaffolds with preserved composition and architecture can be used in tissue engineering to reproduce the complex cardiac extracellular matrix. However, evaluating the extent of cardiomyocyte repopulation of decellularized cardiac extracellular matrix scaffolds after recellularization attempts is challenging. Here, we describe a unique combination of biochemical, biomechanical, histological, and physiological parameters for quantifying recellularization efficiency of tissue-engineered cardiac patches compared with native cardiac tissue. Human embryonic stem cell-derived cardiomyocytes were seeded into rat heart atrial and ventricular decellularized cardiac extracellular matrix patches. Confocal and atomic force microscopy showed cell integration within the extracellular matrix basement membrane that was accompanied by restoration of native cardiac tissue passive mechanical properties. Multi-electrode array and immunostaining (connexin 43) were used to determine synchronous field potentials with electrical coupling. Myoglobin content (~60%) and sarcomere length measurement (>45% vs 2D culture) were used to evaluate cardiomyocyte maturation of integrated cells. The combination of these techniques allowed us to demonstrate that as cellularization efficiency improves, cardiomyocytes mature and synchronize electrical activity, and tissue mechanical/biochemical properties improve toward those of native tissue. © The Author(s) 2020.

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