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Tissue fixation and the effect of molecular fixatives on downstream staining procedures

Authors
  • Howat, William J.
  • Wilson, Beverley A.1, 2, 3
  • 1 Cancer Research UK Cambridge Institute
  • 2 University of Cambridge
  • 3 Li Ka Shing Centre
Type
Published Article
Journal
Methods
Publication Date
Jan 01, 2014
Identifiers
DOI: 10.1016/j.ymeth.2014.01.022
Source
Elsevier
Keywords
License
Unknown

Abstract

It is impossible to underplay the importance of fixation in histopathology. Whether the scientist is interested in the extraction of information on lipids, proteins, RNA or DNA, fixation is critical to this extraction. This review aims to give a brief overview of the current “state of play” in fixation and focus on the effect fixation, and particularly the effect of the newer brand of “molecular fixatives” have on morphology, histochemistry, immunohistochemistry and RNA/DNA analysis. A methodology incorporating the creation of a fixation tissue microarray for the study of the effect of fixation on histochemistry is detailed.

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