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Time of flight versus ion trap MS coupled to CE to analyse intact proteins.

Authors
  • Erny, Guillaume L
  • León, Carlos
  • Marina, Maria Luisa
  • Cifuentes, Alejandro
Type
Published Article
Journal
Journal of Separation Science
Publisher
Wiley (John Wiley & Sons)
Publication Date
May 2008
Volume
31
Issue
10
Pages
1810–1818
Identifiers
DOI: 10.1002/jssc.200700651
PMID: 18481325
Source
Medline
License
Unknown

Abstract

In this work, two different CE-MS instruments, namely, CE-ESI-IT-MS and CE-ESI-TOF-MS, applied to analyse intact proteins from complex samples are investigated. The aim of this work was to compare both instruments in terms of LOD, number of proteins detected, and precision and repeatability in the determination of the protein relative molecular mass. Results show that although CE-ESI-IT-MS provides cleaner MS spectra of intact proteins, CE-ESI-TOF-MS allows the identification of a higher number of proteins from complex matrices in an easier way. Performance in terms of peak area reproducibility, LOD and precision in the determination of the molecular mass were similar for both instruments. The usefulness of the optimised CE-ESI-IT-MS and CE-ESI-TOF-MS conditions was demonstrated by studying the zein-proteins composition of three natural maize lines and their corresponding transgenic lines, showing no significant differences.

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