Affordable Access

Publisher Website

A Time Course Evaluation of Inflammatory and Oxidative Markers Following High Intensity Exercise in Horses: a Pilot Study.

  • MacNicol, Jennifer Laura1
  • Lindinger, Michael Ivan2
  • Pearson, Wendy1
  • 1 1 University of Guelph.
  • 2 2 The Nutraceutical Alliance.
Published Article
Journal of Applied Physiology
American Physiological Society
Publication Date
Oct 26, 2017
DOI: 10.1152/japplphysiol.00461.2017
PMID: 29074709


Exercise is a physiological stress resulting in reactive oxygen species and inflammatory mediators, the accumulation of which are thought to contribute to degenerative articular diseases. The horse is of particular interest in this regard as equine athletes are frequently exposed to repetitive bouts of high intensity exercise. The purpose of this study was to provide a detailed description of the response of articular and systemic oxidative and inflammatory biomarkers following high intensity, exhaustive exercise in horses. A group of horses (Ex) underwent repeated bouts of high intensity exercise, at a target heart rate of 180 bpm, until voluntary exhaustion. Baseline plasma and synovial fluid (SF) samples were taken 24 h prior to exercise, then at 0.5, 1, 2, 4, 8, and 24 h following exercise cessation. This time course was repeated in a group of non-exercised control horses (Co). Plasma and SF samples were analysed for prostaglandin E2 (PGE2), nitric oxide (NO), total antioxidant status (TAS), and glycosaminoglycans (GAG). The Ex group had significantly higher plasma NO at 0.5, 1, and 2 h; and higher plasma PGE2 at 0.5 and 1 h compared to Co. SF PGE2 and GAG were also higher in Ex horses at 8 h compared with Co. It is concluded that high intensity exercise in horses results in a rapid increase in systemic oxidative and inflammatory markers from 0.5-2 h after exercise, which is followed by local articular inflammation and cartilage turnover at 8 h post exercise.

Report this publication


Seen <100 times