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Thymidine kinase-deficient mutants of Aedes albopictus mosquito cells.

Authors
  • Mazzacano, C A
  • Fallon, A M
Type
Published Article
Journal
In Vitro Cellular & Developmental Biology
Publisher
Springer-Verlag
Publication Date
Jun 01, 1992
Volume
28A
Issue
6
Pages
455–458
Identifiers
PMID: 1634444
Source
Medline
License
Unknown

Abstract

Aedes albopictus mosquito cells resistant to the thymidine analog 5-bromodeoxyuridine (BrdU) were obtained using a single-step selection procedure. The resistant cells were characterized with respect to growth in the presence of BrdU, incorporation of [3H]uridine and [3H]thymidine, and thymidine kinase activity in crude extracts. The LC50 for TK-6 cells was 95 micrograms BrdU/ml, and for TK-8 cells was 45 micrograms/ml. Both clones incorporated [3H]uridine at levels corresponding to those in wild-type cells. TK-6 and TK-8 cells did not incorporate [3H]thymidine into acid-precipitable material, nor did they contain measurable thymidine kinase activity. Thymidine kinase activity in crude extracts from wild-type cells had a Km of 2 microM and a Vmax of 10 pmol.min-1.mg-1 protein.

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