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Three new active members of the I-OnuI family of homing endonucleases.

Authors
  • Bilto, Iman M1, 1
  • Guha, Tuhin K1, 1
  • Wai, Alvan1, 1
  • Hausner, Georg1, 1
  • 1 Department of Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada. , (Canada)
Type
Published Article
Journal
Canadian Journal of Microbiology
Publisher
Canadian Science Publishing
Publication Date
Aug 01, 2017
Volume
63
Issue
8
Pages
671–681
Identifiers
DOI: 10.1139/cjm-2017-0067
PMID: 28414922
Source
Medline
Keywords
License
Unknown

Abstract

In vitro characterization of 3 LAGLIDADG-type homing endonucleases (HEs) (I-CcaI, I-CcaII, and I-AstI) that belong to the I-OnuI family showed that they are functional HEs that cleave their respective cognate target sites. These endonucleases are encoded within group ID introns and appear to be orthologues that have inserted into 3 different mitochondrial genes: rns, rnl, and cox3. The endonuclease activity of I-CcaI was tested using various substrates, and its minimum DNA recognition sequence was estimated to be 26 nt. This set of HEs may provide some insight into how these types of mobile elements can migrate into new locations. This study provides additional endonucleases that can be added to the catalog of currently available HEs that may have various biotechnology applications.

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