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Three-dimensional mapping of N-linked oligosaccharides using anion-exchange, hydrophobic and hydrophilic interaction modes of high-performance liquid chromatography.

Authors
  • Takahashi, N1
  • 1 GlycoLab, Nakano Vinegar Co. Ltd., Handa-City, Japan. , (Japan)
Type
Published Article
Journal
Journal of Chromatography A
Publisher
Elsevier
Publication Date
Jan 12, 1996
Volume
720
Issue
1-2
Pages
217–225
Identifiers
PMID: 8601191
Source
Medline
License
Unknown

Abstract

To determine the structure of N-linked oligosaccharides, a three-dimensional (3-D) sugar mapping technique for pyridylaminated neutral and sialyl oligosaccharides is proposed. The pyridylaminated oligosaccharide mixture is first separated by HPLC on a diethylaminoethyl anion-exchange column and the elution data are placed on the Z-axis. Neutral and mono-, di-, tri- and tetrasialyloligosaccharides are then individually separated on both a hydrophobic octadecylsilylsilica column and a hydrophilic amide-silica column under the same conditions as described previously for neutral oligosaccharides. The validity of the 3-D mapping technique was tested using sialyl pyridylaminated oligosaccharides from human serum glycoproteins.

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