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Thiolene-based microfluidic flow cells for surface plasmon resonance imaging.

Authors
Type
Published Article
Journal
Biomicrofluidics
1932-1058
Publisher
American Institute of Physics
Publication Date
Volume
5
Issue
2
Pages
26501–26501
Identifiers
DOI: 10.1063/1.3596395
PMID: 21731900
Source
Medline
License
Unknown

Abstract

Thiolene-based microfluidic devices have been coupled with surface plasmon resonance imaging (SPRI) to provide an integrated platform to study interfacial interactions in both aqueous and organic solutions. In this work, we develop a photolithographic method that interfaces commercially available thiolene resin to gold and glass substrates to generate microfluidic channels with excellent adhesion that leave the underlying sensor surface free from contamination and readily available for surface modification through self-assembly. These devices can sustain high flow rates and have excellent solvent compatibility even with several organic solvents. To demonstrate the versatility of these devices, we have conducted nanomolar detection of streptavidin-biotin interactions using in situ SPRI.

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