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Thioautotrophic ectosymbiosis in Pseudovorticella sp., a peritrich ciliate species colonizing wood falls in marine mangrove.

Authors
  • Grimonprez, Adrien1
  • Molza, Audrey2
  • Laurent, Mélina C Z3
  • Mansot, Jean-Louis4
  • Gros, Olivier5
  • 1 Sorbonne Universités, UPMC Univ Paris 06, Univ Antilles Guyane, Univ Nice Sophia Antipolis, CNRS, Evolution Paris Seine-Institut de Biologie Paris Seine (EPS-IBPS), 75005 Paris, France. Electronic address: [email protected] , (France)
  • 2 GTSI, département de physique, UFR des Sciences Exactes et Naturelles, BP 592, 97159 Pointe-à-Pitre Cedex, Guadeloupe, France. Electronic address: [email protected] , (France)
  • 3 Sorbonne Universités, UPMC Univ Paris 06, Univ Antilles Guyane, Univ Nice Sophia Antipolis, CNRS, Evolution Paris Seine-Institut de Biologie Paris Seine (EPS-IBPS), 75005 Paris, France. Electronic address: [email protected] , (France)
  • 4 GTSI, département de physique, UFR des Sciences Exactes et Naturelles, BP 592, 97159 Pointe-à-Pitre Cedex, Guadeloupe, France; C3MAG, UFR des Sciences Exactes et Naturelles, Université des Antilles, BP 592, 97159 Pointe-à-Pitre, Guadeloupe (French West Indies), France. Electronic address: [email protected] , (France)
  • 5 Sorbonne Universités, UPMC Univ Paris 06, Univ Antilles Guyane, Univ Nice Sophia Antipolis, CNRS, Evolution Paris Seine-Institut de Biologie Paris Seine (EPS-IBPS), 75005 Paris, France; C3MAG, UFR des Sciences Exactes et Naturelles, Université des Antilles, BP 592, 97159 Pointe-à-Pitre, Guadeloupe (French West Indies), France. Electronic address: [email protected] , (France)
Type
Published Article
Journal
European journal of protistology
Publication Date
Feb 01, 2018
Volume
62
Pages
43–55
Identifiers
DOI: 10.1016/j.ejop.2017.11.002
PMID: 29202309
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Ciliates represent a diversified group of protists known to establish symbioses with prokaryotic micro-organisms. They are mainly phagotrophs and symbiotic relationships with bacteria can give them an important advantage in chemosynthetic environments. The aim of this study is to describe the thiotrophic association that occurs between the peritrich ciliate Pseudovorticella sp. and potential sulfur-oxidizing bacteria. Investigations at microscopic scale (LM, SEM, TEM) showed ectosymbiotic bacteria covering the surface of the body of Pseudovorticella sp. According to 16S rDNA phylogenetic analysis, these ectosymbiotic bacteria belong to γ-proteobacteria and are phylogenetically close to the symbiont of the recently described Zoothamnium ignavum, which inhabits shallow-water wood falls. FISH experiments, using symbiont specific probes, clearly indicate that these ectosymbiotic bacteria are also ingested into food vacuoles. Electron lucent granules observed in TEM in the cytoplasm of the ectosymbiotic bacteria have been identified as sulfur granules by Raman microspectrometry analyses. Raman microspectrometry analyses confirmed the thiotrophic nature of this relationship already suggested by the results obtained by TEM and phylogeny. A complete sulfur map was then performed to investigate the sulfur distribution in the zooid. Results show that the relationship between this protist and its bacterial partner is a thiotrophic ectosymbiosis. Copyright © 2017 Elsevier GmbH. All rights reserved.

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