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Thiamine-biosynthesis genes Bbpyr and Bbthi are required for conidial production and cell wall integrity of the entomopathogenic fungus Beauveria bassiana.

Authors
  • Jin, Dan1
  • Sun, Binda2
  • Zhao, Wenqi2
  • Ma, Jincheng3
  • Zhou, Qiuyue1
  • Han, Xuemeng1
  • Mei, Yalin1
  • Fan, Yanhua1
  • Pei, Yan4
  • 1 Biotechnology Research Center, Southwest University, Chongqing, China. , (China)
  • 2 Biotechnology Research Center, Southwest University, Chongqing, China; present address: Institute of Medicine and Hygienic Equipment for High Altitude Region, College of High Altitude Military Medicine, Third Military Medical University, Chongqing, China. , (China)
  • 3 present address: Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, China. , (China)
  • 4 Biotechnology Research Center, Southwest University, Chongqing, China. Electronic address: [email protected] , (China)
Type
Published Article
Journal
Journal of Invertebrate Pathology
Publisher
Elsevier
Publication Date
Jun 14, 2021
Pages
107639–107639
Identifiers
DOI: 10.1016/j.jip.2021.107639
PMID: 34139258
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Beauveria bassiana is an important entomopathogenic fungus used to control a variety of insect pests. Conidia are the infective propagules of the fungus. However, some important factors that influence conidiation are still to be investigated. In this study, a mutant with decreased conidial production and hyphal growth was identified from a random T-DNA insertional library of B. bassiana. The corresponding gene (Bbthi) for this mutation encodes a putative thiazole synthase. Thiazole and pyrimidine are structural components of thiamine (vitamin B1), which is an essential nutrient for all forms of life. Disruption of Bbthi, Bbpyr, a putative pyrimidine synthetic gene, or both in B. bassiana results in a significant decrease of thiamine content. Loss of Bbthi and Bbpyr function significantly decreased the conidial production and hyphal growth, as well as disrupted the integrity of conidial cell wall. However, the defect of Bbpyr and Bbthi does not decrease the virulence of B. bassiana. Our results indicate the importance of thiamine biosynthesis in conidiation of B. bassiana, and provide useful information to produce conidia of entomopathogenic fungi for biocontrol of insect pests. Copyright © 2021. Published by Elsevier Inc.

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