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Thermokinetic responses of the metabolic activity of Staphylococcus lentus cultivated in a glucose limited mineral salt medium

Authors
  • Sekar, Sudharshan1
  • Mahadevan, Surianarayanan1
  • Kumar, Sathish Sundar Dhilip1
  • Mandal, Asit Baran1
  • 1 Central Leather Research Institute (CLRI), Chemical Engineering Department, Adyar, Chennai, Tamilnadu, 600 020, India , Chennai (India)
Type
Published Article
Journal
Journal of Thermal Analysis and Calorimetry
Publisher
Springer-Verlag
Publication Date
Nov 04, 2010
Volume
104
Issue
1
Pages
149–155
Identifiers
DOI: 10.1007/s10973-010-1121-1
Source
Springer Nature
Keywords
License
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Abstract

Biocalorimetric experiments were performed to investigate the metabolic thermal responses of the halotolerant species Staphylococcus lentus in glucose limited mineral salt medium. Growth factors were optimized in both shaker flask and calorimetric experiments. A limiting value of 5 g/L glucose was found to be the optimum for S. lentus growth. The heat flux profiles, OUR, biomass growth, and substrate depletion profiles were compared to deduce the metabolic activity of S. lentus. Shifts in heat flux due to the shifts in substrate uptake and three distinct phases of growth are noticeable in heat profile curves. Respirogram (OUR) and heat profiles are seen to follow the biomass growth curve. Oxycalorific coefficient is validated with the theoretical studies and those noticed in published literature.

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