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The SCFCOI1 Ubiquitin-Ligase Complexes Are Required for Jasmonate Response in Arabidopsis

Authors
Publisher
American Society of Plant Biologists
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PMC
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  • Biology
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Abstract

The SCFCOI1 Ubiquitin-Ligase Complexes Are Required for Jasmonate Response in Arabidopsis by Linghui Xu, Fuquan Liu, Esther Lechner, Pascal Genschik, William L. Crosby, Hong Ma, Wen Peng, Dafang Huang, and Daoxin Xie Plantcell Volume 14(8):1919-1935 August 1, 2002 ©2002 by American Society of Plant Biologists COI1 Interacts with ASK1 and ASK2 in the Yeast Two-Hybrid System.COI1 was fused to the LexA DNA binding domain in pLexA and examined for interaction with ASK1 and ASK2 fused in frame to the activating domain in pB42AD. Xu L et al. Plantcell 2002;14:1919-1935 ©2002 by American Society of Plant Biologists Alignment of Rbx1 Amino Acid Sequences of Arabidopsis, C. elegans, Drosophila, Mouse, and Human.Identical amino acid residues are shaded in black. Xu L et al. Plantcell 2002;14:1919-1935 ©2002 by American Society of Plant Biologists Protein Gel Blot Analysis of Extracts Prepared from the Indicated Arabidopsis Plants.Blots were probed with polyclonal antisera against ASK1 (A), ASK2 (B), Rbx1 (C), or COI1 (D). Xu L et al. Plantcell 2002;14:1919-1935 ©2002 by American Society of Plant Biologists Coimmunoprecipitation Assays.(A) The Flag-tagged COI1 forms an SCF complex in Arabidopsis. Xu L et al. Plantcell 2002;14:1919-1935 ©2002 by American Society of Plant Biologists COI1E22A Abolishes JA Response.coi1-1 plants transgenic for Myc-tagged COI1E22A, Myc-tagged COI1, or vector (see Figure 4C) were examined for JA-inhibitory root growth (top), JA-inducible expression of the defense gene PDF1.2 (middle), and JA-regulated poll... Xu L et al. Plantcell 2002;14:1919-1935 ©2002 by American Society of Plant Biologists Analysis of Glucocorticoid-Inducible AtRbx1(RNAi) Mutant Line A2.(A) DEX treatment resulted in a decrease of both AtRbx1 mRNA and AtRbx1 protein in the glucocorticoid-inducible AtRbx1(RNAi) mutant line A2. Xu L et al. Plantcell 2002;14:1919-1935 ©2002 by American Society of Plant Biologists The Abundance of the Modified AtCUL1 of SCFCOI1 Was Reduced in the axr1-3 Mutan

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