The placental cholinergic system: localization to the cytotrophoblast and modulation of nitric oxide

Affordable Access

The placental cholinergic system: localization to the cytotrophoblast and modulation of nitric oxide

Publisher
BioMed Central
Publication Date
Source
PMC
Keywords
Disciplines
  • Chemistry
License
Unknown

Abstract

1478-811X-4-4.fm ral ss BioMed CentCell Communication and Signaling Open AcceResearch The placental cholinergic system: localization to the cytotrophoblast and modulation of nitric oxide Md Badiul Bhuiyan1, Ferid Murad1 and Michael E Fant*2 Address: 1Department of Integrative Biology and Pharmacology, University of Texas, Health Science Center at Houston, Houston, Texas, 77030, USA and 2Department of Pediatrics, University of Texas Health Science Center at Houston, Houston, Texas, 77030, USA Email: Md Badiul Bhuiyan - [email protected]; Ferid Murad - [email protected]; Michael E Fant* - [email protected] * Corresponding author Abstract Background: The human placenta, a non-neuronal tissue, contains an active cholinergic system comprised of acetylcholine (ACh), choline acetyltransferase (ChAT), acetylcholinesterase (AChE), and high affinity muscarinic receptors. The cell(s) of origin of placental ACh and its role in trophoblast function has not been defined. These studies were performed to define the cellular location of ACh synthesis (ChAT) in the human placenta and to begin studying its functional role. Results: Using immunohistochemical techniques, ChAT was observed primarily within the cytotrophoblasts of preterm placentae as well as some mesenchymal elements. Similar intense immunostaining of the cytotrophoblast was observed for endothelium-derived nitric oxide synthase (eNOS) suggesting that ACh may interact with nitric oxide (NO)-dependent signaling pathways. The ability of carbamylcholine (CCh), an ACh analogue, to stimulate a rise in intracellular Ca++ and NO production in trophoblasts was therefore tested using the BeWob30 choriocarcinoma cell as a model system. First, CCh significantly increased intracellular calcium as assessed by fluorescence microscopy. We then examined the ability of CCh to stimulate NO production by measuring total nitrite/nitrate production in conditioned media using chemiluminescence-based analysis. CCh, alone

Statistics

Seen <100 times