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The Phosphoprotein (P) Binding Site Resides in the N Terminus of the L Polymerase Subunit of Sendai Virus

Authors
  • David E. Holmes
  • Sue A. Moyer
Publisher
American Society for Microbiology
Publication Date
Mar 01, 2002
Source
PMC
Keywords
Disciplines
  • Biology
License
Unknown

Abstract

Sendai virus encodes an RNA-dependent RNA polymerase which is composed of the L and P proteins. Site-directed mutagenesis of the N terminus of L has identified amino acids important for binding P. Seven of nine mutants in amino acids 1 to 350 of Sendai L lost the ability to bind to Sendai P, although they were still able to bind the viral C protein. Loss of P binding correlated with the loss of all RNA synthesis activities. Two L mutants gave limited P-L complex formation and limited viral transcription and replication.

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