The 3' noncoding region of beta-globin mRNA is not essential for in vitro translation.
- Authors
- Publication Date
- Jan 01, 1979
- Source
- PMC
- Keywords
- License
- Unknown
Abstract
Rabbit beta globin DNA sequence, excised from plasmid pbetaG1, directs in vitro synthesis of beta-globin in a transcription-translation cell-free system, even after specific elimination of the entire 3'-noncoding region. A DNA restriction fragment carrying this 3' noncoding region and hybridized to globin mRNA cannot arrest the cell-free translation of beta-globin mRNA.