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Testosterone metabolism in male rat epiphysis.

Authors
  • Ackerman, R J Jr
  • Hamilton, D W
Type
Published Article
Journal
Calcified tissue research
Publication Date
Apr 13, 1976
Volume
20
Issue
1
Pages
31–40
Identifiers
PMID: 4200
Source
Medline
License
Unknown

Abstract

Using radioactive substrate, thin-layer chromatography and recrystallization methods as well as differential centrifugation, gel filtration and electrophoresis, testosterone metabolism was investigated in male rat epiphyseal growth plate. 5alpha-androstane 3alpha-17beta-diol was found to be a major metabolite in vitro; lesser amounts of androstenedione and androstanedione, and a very small amount of 5alpha-dihydrotestosterone were also identified. Radioactivity was recovered in epiphyseal subcellular fractions 30 minutes following in vivo administration of tritiated 5alpha-dihydrotestosterone; within 2 hours radioactivity had fallen to essentially background level in all fractions but the cytosol. A testosterone binding protein could not be identified within epiphyseal cytosol.

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