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Testicular expression of TDRD1, TDRD5, TDRD9 and TDRD12 in azoospermia

Authors
  • Babakhanzadeh, Emad1, 1
  • Khodadadian, Ali1
  • Rostami, Saadi2
  • Alipourfard, Iraj3, 4
  • Aghaei, Mohsen1
  • Nazari, Majid1
  • Hosseinnia, Mehdi5
  • Mehrjardi, Mohammad Yahya Vahidi1, 1
  • Jamshidi, Yalda6
  • Ghasemi, Nasrin1, 7
  • 1 Shahid Sadoughi University of Medical Sciences, Yazd, Iran , Yazd (Iran)
  • 2 Azarbaijan Shahid Madani University, Tabriz, Iran , Tabriz (Iran)
  • 3 University of Vienna, Vienna, Austria , Vienna (Austria)
  • 4 University of Rome Tor Vergata, Rome, Italy , Rome (Italy)
  • 5 University of Guilan, Rasht, Iran , Rasht (Iran)
  • 6 St George’s University of London, London, UK , London (United Kingdom)
  • 7 Shahid sadoughi University of Medical Sciences, Yazd, Iran , Yazd (Iran)
Type
Published Article
Journal
BMC Medical Genetics
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Feb 14, 2020
Volume
21
Issue
1
Identifiers
DOI: 10.1186/s12881-020-0970-0
Source
Springer Nature
Keywords
License
Green

Abstract

BackgroundTudor domain-containing proteins (TDRDs) play a critical role in piRNA biogenesis and germ cell development. piRNAs, small regulatory RNAs, act by silencing of transposons during germline development and it has recently been shown in animal model studies that defects in TDRD genes can lead to sterility in males.MethodsHere we evaluate gene and protein expression levels of four key TDRDs (TDRD1, TDRD5, TDRD9 and TDRD12) in testicular biopsy samples obtained from men with obstructive azoospermia (OA, n = 29), as controls, and various types of non-obstructive azoospermia containing hypospermatogenesis (HP, 28), maturation arrest (MA, n = 30), and Sertoli cell-only syndrome (SCOS, n = 32) as cases. One-way ANOVA test followed by Dunnett’s multiple comparison post-test was used to determine inter-group differences in TDRD gene expression among cases and controls.ResultsThe results showed very low expression of TDRD genes in SCOS specimens. Also, the expression of TDRD1 and TDRD9 genes were lower in MA samples compared to OA samples. The expression of TDRD5 significantly reduced in SCOS, MA and HP specimens than the OA specimens. Indeed, TDRD12 exhibited a very low expression in HP specimens in comparison to OA specimens. All these results were confirmed by Western blot technique.ConclusionTDRDs could be very important in male infertility, which should be express in certain stages of spermatogenesis.

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