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A ternary AppA-PpsR-DNA complex mediates light regulation of photosynthesis-related gene expression.

Authors
  • Winkler, Andreas
  • Heintz, Udo
  • Lindner, Robert
  • Reinstein, Jochen
  • Shoeman, Robert L
  • Schlichting, Ilme
Type
Published Article
Journal
Nature Structural & Molecular Biology
Publisher
Springer Nature
Publication Date
Jul 01, 2013
Volume
20
Issue
7
Pages
859–867
Identifiers
DOI: 10.1038/nsmb.2597
PMID: 23728293
Source
Medline
License
Unknown

Abstract

The anoxygenic phototrophic bacterium Rhodobacter sphaeroides uses different energy sources, depending on environmental conditions including aerobic respiration or, in the absence of oxygen, photosynthesis. Photosynthetic genes are repressed at high oxygen tension, but at intermediate levels their partial expression prepares the bacterium for using light energy. Illumination, however, enhances repression under semiaerobic conditions. Here, we describe molecular details of two proteins mediating oxygen and light control of photosynthesis-gene expression: the light-sensing antirepressor AppA and the transcriptional repressor PpsR. Our crystal structures of both proteins and their complex and hydrogen/deuterium-exchange data show that light activation of AppA-PpsR2 affects the PpsR effector region within the complex. DNA binding studies demonstrate the formation of a light-sensitive ternary AppA-PpsR-DNA complex. We discuss implications of these results for regulation by light and oxygen, highlighting new insights into blue light-mediated signal transduction.

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