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Terminal segment of Kluyveromyces lactis linear DNA plasmid pGKL2 supports autonomous replication of hybrid plasmids in Saccharomyces cerevisiae.

Authors
  • Fujimura, H
  • Hishinuma, F
  • Gunge, N
Type
Published Article
Journal
Current genetics
Publication Date
Jan 01, 1987
Volume
12
Issue
2
Pages
99–104
Identifiers
PMID: 3449223
Source
Medline
License
Unknown

Abstract

By use of linear DNA plasmid pGKL2 from the yeast Kluyveromyces lactis we have constructed hybrid plasmids carrying a LEU2 gene of Saccharomyces cerevisiae as a selectable marker. The replication properties of hybrid plasmids in yeasts were investigated. We demonstrated that the insertion of a LEU2 gene into pGKL2 resulted in circularization of the hybrid plasmids and pGKL2 segment supported autonomous replication of the plasmids. Moreover, the hybrid plasmids propagated autonomously, independently of the presence of the natural pGKL2 plasmid.

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