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Temporal analyses of postnatal liver development and maturation by single-cell transcriptomics.

Authors
  • Liang, Yan1
  • Kaneko, Kota1
  • Xin, Bing1
  • Lee, Jin1
  • Sun, Xin2
  • Zhang, Kun3
  • Feng, Gen-Sheng4
  • 1 Department of Pathology, Division of Biological Sciences, and Moores Cancer Center, University of California, San Diego, La Jolla, CA 92093, USA.
  • 2 Department of Pediatrics, University of California, San Diego, La Jolla, CA 92093, USA.
  • 3 Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA.
  • 4 Department of Pathology, Division of Biological Sciences, and Moores Cancer Center, University of California, San Diego, La Jolla, CA 92093, USA. Electronic address: [email protected]
Type
Published Article
Journal
Developmental cell
Publication Date
Feb 07, 2022
Volume
57
Issue
3
Identifiers
DOI: 10.1016/j.devcel.2022.01.004
PMID: 35134346
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The postnatal development and maturation of the liver, the major metabolic organ, are inadequately understood. We have analyzed 52,834 single-cell transcriptomes and identified 31 cell types or states in mouse livers at postnatal days 1, 3, 7, 21, and 56. We observe unexpectedly high levels of hepatocyte heterogeneity in the developing liver and the progressive construction of the zonated metabolic functions from pericentral to periportal hepatocytes, which is orchestrated with the development of sinusoid endothelial, stellate, and Kupffer cells. Trajectory and gene regulatory analyses capture 36 transcription factors, including a circadian regulator, Bhlhe40, in programming liver development. Remarkably, we identified a special group of macrophages enriched at day 7 with a hybrid phenotype of macrophages and endothelial cells, which may regulate sinusoidal construction and Treg-cell function. This study provides a comprehensive atlas that covers all hepatic cell types and is instrumental for further dissection of liver development, metabolism, and disease. Copyright © 2022 Elsevier Inc. All rights reserved.

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