Affordable Access

Temperature induced interstrand crosslinks in cisplatin-DNA adducts detected by electrophoresis and UV spectrophotometer.

Authors
  • Narasimhan, V
  • Malboueuf, B
  • Hodil, S E
Type
Published Article
Journal
Biochemistry and molecular biology international
Publication Date
Nov 01, 1995
Volume
37
Issue
5
Pages
843–851
Identifiers
PMID: 8624489
Source
Medline
License
Unknown

Abstract

Thermal melting profiles of DNA samples complexed with the anti-tumor drug cisplatin exhibit significant hypochromicity at pre-melting temperatures while a reduction in total hyperchromicity is observed in the melting region. Densitometric analysis of agarose gel electrophoresis pattern of DNA-cisplatin adducts heated to different temperatures in the pre-melting region and frozen in their conformations reveals a gradual retardation of mobility as the temperature increases. We attribute these results to a temperature induced transition in the mode of binding of cisplatin to DNA from an initial intrastrand monofunctional binding to bifunctional interstrand crosslink formation which results in gradual bending of the helix.

Report this publication

Statistics

Seen <100 times