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A technology platform for the fast production of monoclonal recombinant antibodies against plant proteins and peptides.

Authors
  • Eeckhout, Dominique
  • De Clercq, Annelies
  • Van De Slijke, Eveline
  • Van Leene, Jelle
  • Stals, Hilde
  • Casteels, Peter
  • Persiau, Geert
  • Vercammen, Dominique
  • Van Breusegem, Frank
  • Zabeau, Marc
  • Inzé, Dirk
  • Jespers, Laurent
  • Depicker, Ann
  • De Jaeger, Geert
Type
Published Article
Journal
Journal of Immunological Methods
Publisher
Elsevier
Publication Date
Nov 01, 2004
Volume
294
Issue
1-2
Pages
181–187
Identifiers
PMID: 15604026
Source
Medline
License
Unknown

Abstract

The application of recombinant antibodies in plant biology research is limited because plant researchers have minimal access to high-quality phage display libraries. Therefore, we constructed a library of 1.3 x 10(10) clones displaying human single-chain variable fragments (scFvs) that is available to the academic community. The scFvs selected from the library against a diverse set of plant proteins showed moderate to high antigen-binding affinity together with high specificity. Moreover, to optimize an scFv as immunodetection agent, two expression systems that allow efficient production and purification of bivalent scFv-Fc and scFv-CkappaZIP fusion proteins were integrated. We are convinced that this antibody platform will further stimulate applications of recombinant antibodies such as the diagnostic detection or immunomodulation of specific antigens in plants.

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