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Techniques for the optimum recovery of cold injured Campylobacter jejuni from milk or water.

Authors
  • Humphrey, T J
Type
Published Article
Journal
The Journal of applied bacteriology
Publication Date
Aug 01, 1986
Volume
61
Issue
2
Pages
125–132
Identifiers
PMID: 3771411
Source
Medline
License
Unknown

Abstract

When broth was inoculated with cells of Campylobacter jejuni that had been injured by chilling there was a fall in the viable population of up to 90%. It was greater at 43 degrees than 37 degrees C and in the presence of certain antibiotics and in some cases resulted in a surviving population that was below the minimum inoculum for subsequent growth. A technique of pre-enrichment in non-selective culture broth at 37 degrees C for 2 h before the addition of antibiotics and incubation at 43 degrees C was found to significantly reduce the fall in numbers and to improve the detection of C. jejuni in samples of raw milk and water.

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