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Technical report: laser microdissection of cochlear structures from celloidin embedded human temporal bone tissues and detection of the mitochondrial DNA common deletion using real time PCR.

Authors
  • Markaryan, Adam1
  • Nelson, Erik G
  • Tretiakova, Maria
  • Hinojosa, Raul
  • 1 Section of Otolaryngology - Head and Neck Surgery, Department of Surgery, University of Chicago, 5841 S. Maryland Avenue, MC 1035, Chicago, IL 60637, United States. [email protected] , (United States)
Type
Published Article
Journal
Hearing Research
Publisher
Elsevier BV
Publication Date
October 2008
Volume
244
Issue
1-2
Pages
1–6
Identifiers
DOI: 10.1016/j.heares.2008.07.007
PMID: 18706496
Source
Medline
License
Unknown

Abstract

Laser microdissection (LMD) has been used to isolate groups of cells and single cells from numerous tissues. In this study, we describe a technique for isolating cochlear structures and individual spiral ganglion cells from archival celloidin embedded human temporal bone sections. The specimens isolated are suitable for quantifying the mitochondrial DNA (mtDNA) common deletion (CD) within these tissues using a real time polymerase chain reaction (PCR) assay. The results presented in this manuscript demonstrate the feasibility of using this LMD technique to study the accumulation of mtDNA deletions in diseases of the ear. To our knowledge, this approach to analyzing archival human temporal bone tissues has not been previously reported.

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