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Technetium labeling of monoclonal antibodies with functionalized BATOs: 2. TcCl(DMG)3CPITC labeling of B72.3 and NP-4 whole antibodies and NP-4 F(ab')2.

Authors
  • Linder, K E
  • Wen, M D
  • Nowotnik, D P
  • Ramalingam, K
  • Sharkey, R M
  • Yost, F
  • Narra, R K
  • Nunn, A D
  • Eckelman, W C
Type
Published Article
Journal
Bioconjugate chemistry
Publication Date
Jan 01, 1991
Volume
2
Issue
6
Pages
407–414
Identifiers
PMID: 1805937
Source
Medline
License
Unknown

Abstract

BATO (boronic acid adduct of technetium dioximes) complexes, TcCl(dioxime)3BR, were prepared in which the boron substituent (R) was the protein-reactive 2-carboxy-4-phenyl isothiocyanate (CPITC). The 99Tc complexes, where the dioxime was either dimethylglyoxime (DMG) or cyclohexanedione dioxime (CDO), were prepared and characterized. The 99mTc complex TcCl(DMG)3CPITC was prepared from a freeze-dried kit and used to label B72.3 (anti-TAG.72) and NP-4 (anti-CEA) whole antibodies, and the NP-4 F(ab')2 fragment. SDS-PAGE electrophoresis indicated that the labeling reagent was strongly bound to antibody. The labeled antibodies displayed high binding to affinity columns and good tumor uptake in GW39 tumor-bearing mice.

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