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Tea polyphenol protects against cisplatin-induced meiotic defects in porcine oocytes.

Authors
  • Zhou, Changyin1
  • Zhang, Xue1
  • ShiYang, Xiayan1
  • Wang, Huili2
  • Xiong, Bo1
  • 1 College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. , (China)
  • 2 Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China. , (China)
Type
Published Article
Journal
Aging
Publisher
"Impact Journals, LLC "
Publication Date
Jul 13, 2019
Volume
11
Issue
13
Pages
4706–4719
Identifiers
DOI: 10.18632/aging.102084
PMID: 31301169
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

DDP (cisplatin), a DNA cross-linking agent, is one of the most common chemotherapeutic drugs that have been widely used in the treatment of sarcomas and germ cell tumors. DDP treatment exhibits severe side effects including renal toxicity, ototoxicity and embryo-toxicity. Women of reproductive age treated with DDP may lead to loss of primordial follicles, resulting in the depletion of the ovarian reserve and consequent premature ovarian failure. However, the influence of DDP on the oocyte quality and the strategy to prevent it has not yet fully clarified. Here, we report that DDP exposure resulted in the oocyte meiotic failure via disrupting the meiotic organelle dynamics and arrangement, exhibiting a prominently impaired cytoskeleton assembly, including spindle formation and actin polymerization. In addition, exposure to DDP led to the abnormal distribution of mitochondrion and cortical granules, two indicators of cytoplasmic maturation of oocytes. Conversely, TP (tea polyphenols) supplementation partially restored all of the meiotic defects resulted from DDP exposure through suppressing the increase of ROS level and the occurrence of DNA damage as well as apoptosis.

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