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Taxol-induced bcl-2 phosphorylation in ovarian cancer cell monolayer and spheroids.

Authors
  • Makhija, S
  • Taylor, D D
  • Gibb, R K
  • Gerçel-Taylor, C
Type
Published Article
Journal
International journal of oncology
Publication Date
Mar 01, 1999
Volume
14
Issue
3
Pages
515–521
Identifiers
PMID: 10024685
Source
Medline
License
Unknown

Abstract

Expression of Bcl-2, Bax, p53 and induction of apoptosis were studied in cisplatin or Taxol treated monolayer and spheroid cultures of ovarian cancer cell lines (SKOV-3, UL-1, UL-3C). While cisplatin (15-75 microg/ml) induced apoptosis in monolayer and spheroid cultures, Taxol (100-800 nM) induced fragmentation in monolayers only. Cisplatin induced up to 5-fold DNA fragmentation in monolayers, while 3-fold (UL-3C, SKOV-3), and 1.5-fold (UL-1) in spheroids. Taxol treatment of monolayers resulted in the characteristic phosphorylation of Bcl-2, which was not demonstrated in spheroid cultures. Bax expression was reduced in spheroids following cisplatin or Taxol treatment, while p53 levels remained unchanged.

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