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Taurine suppresses osteoblastic differentiation of aortic valve interstitial cells induced by beta-glycerophosphate disodium, dexamethasone and ascorbic acid via the ERK pathway

Authors
  • Feng, Xiang1
  • Li, Jian-ming1
  • Liao, Xiao-bo1
  • Hu, Ye-rong1
  • Shang, Bao-peng1
  • Zhang, Zhi-yuan1
  • Yuan, Ling-qing2
  • Xie, Hui2
  • Sheng, Zhi-feng2
  • Tang, Hao1
  • Zhang, Wei1
  • Gu, Lu1
  • Zhou, Xin-min1
  • 1 The Second Xiang-Ya Hospital, Central South University, Department of Cardiothoracic Surgery, Changsha, Hunan, 410011, People’s Republic of China , Changsha (China)
  • 2 The Second Xiang-Ya Hospital, Central South University, Institute of Metabolism and Endocrinology, Changsha, Hunan, 410011, People’s Republic of China , Changsha (China)
Type
Published Article
Journal
Amino Acids
Publisher
Springer-Verlag
Publication Date
Mar 01, 2012
Volume
43
Issue
4
Pages
1697–1704
Identifiers
DOI: 10.1007/s00726-012-1253-7
Source
Springer Nature
Keywords
License
Yellow

Abstract

Aortic valve calcification (AVC) is an active process characterized by osteoblastic differentiation of the aortic valve interstitial cells (AVICs). Taurine is a free β-amino acid and plays important physiological roles including protective effect of cardiovascular events. To evaluate the possible role of taurine in AVC, we isolated human AVICs from patients with type A dissection without leaflet disease. We demonstrated that the cultured AVICs express SM α-actin, vimentin and taurine transporter (TAUT), but not CD31, SM-myosin or desmin. We also established the osteoblastic differentiation model of the AVICs induced by pro-calcific medium (PCM) containing β-glycerophosphate disodium, dexamethasone and ascorbic acid in vitro. The results showed that taurine attenuated the PCM-induced osteoblastic differentiation of AVICs by decreasing the alkaline phosphate (ALP) activity/expression and the expression of the core binding factor α1 (Cbfα1) in a dose-dependent manner (reaching the maximum protective effect at 10 mM), and taurine (10 mM) inhibited the mineralization level of AVICs in the form of calcium content significantly. Furthermore, taurine activated the extracellular signal-regulated protein kinase (ERK) pathway via TAUT, and the inhibitor of ERK (PD98059) abolished the effect of taurine on both ALP activity/expression and Cbfα1 expression. These results suggested that taurine could inhibit osteoblastic differentiation of AVIC via the ERK pathway.

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