Affordable Access

Access to the full text

Targeting the hallmarks of cancer: the effects of silibinin on proliferation, cell death, angiogenesis, and migration in colorectal cancer

Authors
  • Sameri, Saba1
  • Mohammadi, Chiman1
  • Mehrabani, Mehrnaz2
  • Najafi, Rezvan1
  • 1 Hamadan University of Medical Sciences, Hamadan, Iran , Hamadan (Iran)
  • 2 Kerman University of Medical Sciences, Kerman, Iran , Kerman (Iran)
Type
Published Article
Journal
BMC Complementary Medicine and Therapies
Publisher
BioMed Central
Publication Date
May 31, 2021
Volume
21
Issue
1
Identifiers
DOI: 10.1186/s12906-021-03330-1
Source
Springer Nature
Keywords
License
Green

Abstract

BackgroundSilibinin, as a chemopreventive agent, has shown anti-cancer efficacy against different types of cancers. In the present study, we investigated the anti-cancer activities of silibinin on CT26 mouse colon cell line.MethodsCT26 cells were treated with different concentrations of silibinin. To examine the cytotoxic effect of silibinin on proliferation, apoptosis, autophagy, angiogenesis, and migration, MTT, colony-forming assay, Annexin V/PI flow cytometry, RT-qPCR, and Scratch assay were used.ResultsSilibinin was found to significantly reduce CT26 cells survival. Furthermore, silibinin strongly induced apoptosis and autophagy by up-regulating the expression of Bax, Caspase-3, Atg5, Atg7 and BECN1 and down-regulating Bcl-2. Silibinin considerably down-regulated the expression of COX-2, HIF-1α, VEGF, Ang-2, and Ang-4 as well as the expression of MMP-2, MMP-9, CCR-2 and CXCR-4.ConclusionsThe present study revealed that silibinin shows anticancer activities by targeting proliferation, cell survival, angiogenesis, and migration of CT26 cells.

Report this publication

Statistics

Seen <100 times