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Targeted Perturb-seq enables genome-scale genetic screens in single cells

Authors
  • Schraivogel, Daniel1
  • Gschwind, Andreas R.2
  • Milbank, Jennifer H.1
  • Leonce, Daniel R.1
  • Jakob, Petra1
  • Mathur, Lukas1
  • Korbel, Jan O.1
  • Merten, Christoph A.1
  • Velten, Lars1, 3
  • Steinmetz, Lars M.1, 2, 4
  • 1 European Molecular Biology Laboratory (EMBL), Genome Biology Unit, Heidelberg, Germany , Heidelberg (Germany)
  • 2 Stanford University School of Medicine, Stanford, CA, USA , Stanford (United States)
  • 3 Center for Genomic Regulation, The Barcelona Institute of Science and Technology, Barcelona, Spain , Barcelona (Spain)
  • 4 Stanford Genome Technology Center, Palo Alto, CA, USA , Palo Alto (United States)
Type
Published Article
Journal
Nature Methods
Publisher
Springer Nature
Publication Date
Jun 01, 2020
Volume
17
Issue
6
Pages
629–635
Identifiers
DOI: 10.1038/s41592-020-0837-5
Source
Springer Nature
License
Yellow

Abstract

Targeted sequencing of perturbation effects offers a sensitive approach to capture genes of interest in CRISPR-mediated screens, enabling genome-scale screens at higher scale and lower cost than whole-transcriptome Perturb-seq.

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