Affordable Access

deepdyve-link
Publisher Website

Targeted amelioration of cisplatin-induced ototoxicity in guinea pigs.

Authors
  • Mohan, Shaulnie1
  • Smyth, Brendan J2
  • Namin, Arya3
  • Phillips, Grady3
  • Gratton, Michael Anne3
  • 1 Department of Otolaryngology-Head & Neck Surgery, Saint Louis University, Saint Louis, Missouri, USA [email protected]
  • 2 Baxter Health Care Corporation, Deerfield, Illinois, USA.
  • 3 Department of Otolaryngology-Head & Neck Surgery, Saint Louis University, Saint Louis, Missouri, USA.
Type
Published Article
Journal
Otolaryngology
Publisher
SAGE Publications
Publication Date
Nov 01, 2014
Volume
151
Issue
5
Pages
836–839
Identifiers
DOI: 10.1177/0194599814544877
PMID: 25078416
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

This pilot study compared otoprotection provided by trans-tympanic formulations and systemic intraperitoneal administration of L-N-acetylcysteine from cisplatin-induced cochlear oxidative stress. Protection was assessed by measures of hearing loss and cochlear glutathione levels. All groups received an equivalent single dose of L-N-acetylcysteine followed by cisplatin. Cisplatin was administered subcutaneously for 3 days (5.5 mg/kg/day). Two hours prior to day 1 cisplatin, L-N-acetylcysteine was administered either intraperitoneally (250 mg/kg), trans-tympanic as 2% L-N-acetylcysteine in gel, or trans-tympanic as L-N-acetylcysteine-loaded nanocapsules in gel. Hearing was assessed prior to and 3 days after cisplatin followed by microdissection of cochlear tissue. The levels of reduced (GSH) and oxidized (GSSG) glutathione in homogenized tissue supernatants were determined via luminometry. Intraperitoneal L-N-acetylcysteine administration preceding cisplatin resulted in less hearing loss and a higher GSH/GSSG ratio than either trans-tympanic formulation. This suggests that for equivalent doses of L-N-acetylcysteine, systemic rather than targeted cochlear delivery provides increased otoprotection from cisplatin ototoxicity. © American Academy of Otolaryngology—Head and Neck Surgery Foundation 2014.

Report this publication

Statistics

Seen <100 times