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TaqMan probe-based real-time PCR assay for detection and discrimination of class I, II, and III tfdA genes in soils treated with phenoxy acid herbicides.

Authors
  • Baelum, Jacob1
  • Jacobsen, Carsten S
  • 1 Geological Survey of Denmark and Greenland (GEUS), Department of Geochemistry, Øster Voldgade 10, DK-1350 Copenhagen K, Denmark. , (Denmark)
Type
Published Article
Journal
Applied and Environmental Microbiology
Publisher
American Society for Microbiology
Publication Date
May 01, 2009
Volume
75
Issue
9
Pages
2969–2972
Identifiers
DOI: 10.1128/AEM.02051-08
PMID: 19251892
Source
Medline
License
Unknown

Abstract

Separate quantification of three classes of tfdA genes was performed using TaqMan quantitative real-time PCR for 13 different soils subsequent to mineralization of three phenoxy acids. Class III tfdA genes were found to be involved in mineralization more often than class I and II tfdA genes.

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