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Tanshinone IIA Induces Heme Oxygenase 1 Expression and Inhibits Cyclic Strain-Induced Interleukin 8 Expression in Vascular Endothelial Cells.

Authors
  • Zhuang, Shaowei1
  • Cheng, Tzu-Hurng2
  • Shih, Nang-Lang3
  • Liu, Ju-Chi4
  • Chen, Jin-Jer5, 6
  • Hong, Hong-Jye7
  • Chan, Paul8, 9
  • 1 * Deparment of Cardiology, Shanghai East Hospital.
  • 2 ‡ Department of Biochemistry, School of Medicine, College of Medicine.
  • 3 ¶ Department of Life Sciences, National University of Kaohsiung, Kaohsiung, Taiwan, R.O.C. , (Taiwan)
  • 4 ∥ Division of Cardiology, Department of Internal Medicine, Shuang Ho Hospital Taipei Medical University, New Taipei City, Taiwan, R.O.C. , (Taiwan)
  • 5 ** Graduate Institute of Clinical Medicine, College of Medicine, China Medical University Hospital, Taiwan, R.O.C. , (China)
  • 6 †† Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, R.O.C. , (Taiwan)
  • 7 § School of Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung, Taiwan, R.O.C. , (China)
  • 8 † Shanghai East Taiwanese Hospital, Tongji University, Shanghai, P.R. China. , (China)
  • 9 ‡‡ Deparment of Cardiology, Taipei Medical University-Wan Fang Hospital, Taipei, Taiwan, R.O.C. , (Taiwan)
Type
Published Article
Journal
The American journal of Chinese medicine
Publication Date
Jan 01, 2016
Volume
44
Issue
2
Pages
377–388
Identifiers
DOI: 10.1142/S0192415X1650021X
PMID: 27080946
Source
Medline
Keywords
License
Unknown

Abstract

Tanshinone IIA is the main effective component of Salvia miltiorrhiza, known as "Danshen," which has been used in many therapeutic remedies in traditional Chinese medicine. However, the direct effects of tanshinone IIA on vascular endothelial cells have not yet been fully described. In the present study, we demonstrated that tanshinone IIA increased heme oxygenase-1 (HO-1) expression in human umbilical vein endothelial cells. Western blot analyses and experiments with specific inhibitors indicated tanshinone IIA enhanced HO-1 expression through the activation of phosphoinositide 3-kinase (PI3K)/Akt and the subsequent induction of nuclear factor erythroid 2-related factor 2 (Nrf2) nuclear translocation. In addition, tanshinone IIA inhibited cyclic strain induced interleukin-8 (IL-8) expression. HO-1 silencing significantly abrogated the repressive effects of tanshinone IIA on strain-induced IL-8 expression, which suggests HO-1 has a role in mediating the effects of tanshinone IIA. This study reports for the first time that tanshinone IIA inhibits cyclic strain-induced IL-8 expression via the induction of HO-1 in endothelial cells, providing valuable new insight into the molecular pathways that may contribute to the effects of tanshinone IIA.

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