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Talin and signaling through integrins.

Authors
  • Bouaouina, Mohamed
  • Harburger, David S
  • Calderwood, David A
Type
Published Article
Journal
Methods in Molecular Biology
Publication Date
Jan 01, 2012
Volume
757
Pages
325–347
Identifiers
DOI: 10.1007/978-1-61779-166-6_20
PMID: 21909921
Source
Medline
License
Unknown

Abstract

Integrin adhesion receptors are essential for the development and functioning of multicellular animals. Integrins mediate cell adhesion to the extracellular matrix and to counter-receptors on adjacent cells, and the ability of integrins to bind extracellular ligands is regulated in response to intracellular signals that act on the short cytoplasmic tails of integrin subunits. Integrin activation, the rapid conversion of integrin receptors from low to high affinity, requires binding of talin to integrin β tails and, once bound, talin provides a connection from activated integrins to the actin cytoskeleton. A wide range of experimental approaches have contributed to the current understanding of the importance of talin in integrin signaling. Here, we describe two methods that have been central to our investigations of talin; a biochemical assay that has allowed characterization of interactions between integrin cytoplasmic tails and talin, and a fluorescent-activated cell-sorting procedure to assess integrin activation in cultured cells expressing talin domains, mutants, dominant negative constructs, or shRNA.

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