T Cell Repertoire Diversity Is Decreased in Type 1 Diabetes Patients.
Department of Biology, South University of Science and Technology of China, Shenzhen 518055, China.
Department of Endocrinology, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China.
Department of Gastrointestinal Surgery, Shenzhen People's Hospital, the Second Clinical Medical College of Jinan University, Shenzhen 518020, China.
Department of Infectious Diseases and Hepatology Unit, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Departments of Bioengineering and Physics & Astronomy, Rice University, Houston, TX 77005, USA.
Shenzhen Tumor Immune-Gene Therapy Clinical Application Engineering Lab, Biobank of the Second People's Hospital, the First Affiliated Hospital of Shenzhen University, Shenzhen 518035, China. Electronic address: [email protected]
Department of Biology, South University of Science and Technology of China, Shenzhen 518055, China. Electronic address: [email protected]
- Published Article
Genomics, proteomics & bioinformatics
- Publication Date
Dec 23, 2016
Type 1 diabetes mellitus (T1D) is an immune-mediated disease. The autoreactive T cells in T1D patients attack and destroy their own pancreatic cells. In order to systematically investigate the potential autoreactive T cell receptors (TCRs), we used a high-throughput immune repertoire sequencing technique to profile the spectrum of TCRs in individual T1D patients and controls. We sequenced the T cell repertoire of nine T1D patients, four type 2 diabetes (T2D) patients, and six nondiabetic controls. The diversity of the T cell repertoire in T1D patients was significantly decreased in comparison with T2D patients (P=7.0E-08 for CD4+ T cells, P=1.4E-04 for CD8+ T cells) and nondiabetic controls (P=2.7E-09 for CD4+ T cells, P=7.6E-06 for CD8+ T cells). Moreover, T1D patients had significantly more highly-expanded T cell clones than T2D patients (P=5.2E-06 for CD4+ T cells, P=1.9E-07 for CD8+ T cells) and nondiabetic controls (P=1.7E-07 for CD4+ T cells, P=3.3E-03 for CD8+ T cells). Furthermore, we identified a group of highly-expanded T cell receptor clones that are shared by more than two T1D patients. Although further validation in larger cohorts is needed, our data suggest that T cell receptor diversity measurements may become a valuable tool in investigating diabetes, such as using the diversity as an index to distinguish different types of diabetes.
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The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/28024918