Affordable Access

deepdyve-link
Publisher Website

The T cell receptor (TRA) locus in the rabbit (Oryctolagus cuniculus): Genomic features and consequences for invariant T cells.

Authors
  • Mondot, Stanislas1
  • Lantz, Olivier2, 3, 4
  • Lefranc, Marie-Paule5
  • Boudinot, Pierre6
  • 1 MICALIS, Institut National de la Recherche Agronomique (INRA), Université Paris-Saclay, Jouy-en-Josas, France. , (France)
  • 2 INSERM U932, Institut Curie, Paris Sciences et Lettres Research University, Paris, France. , (France)
  • 3 Center of Clinical Investigation in Biotherapy 1428, Gustave-Roussy/Curie, Paris, France. , (France)
  • 4 Laboratoire d'Immunologie Clinique, Institut Curie, Paris, France. , (France)
  • 5 IMGT®, the International ImMunoGeneTics Information System® (IMGT), Institut de Génétique Humaine, CNRS, Université de Montpellier, Montpellier Cedex 5, France. , (France)
  • 6 Virologie et Immunologie Moléculaires (VIM), Institut National de la Recherche Agronomique (INRA), Université Paris-Saclay, Jouy-en-Josas, France. , (France)
Type
Published Article
Journal
European Journal of Immunology
Publisher
Wiley (John Wiley & Sons)
Publication Date
Dec 01, 2019
Volume
49
Issue
12
Pages
2146–2158
Identifiers
DOI: 10.1002/eji.201948228
PMID: 31355919
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The rabbit has been widely used in immunology and infectiology. Rabbit immunoglobulins have been extensively studied, leading to the discovery of their idiotypes, allotypic diversity, and of the diversification of the primary repertoire by hyperconversion. Much less is known about rabbit T cell receptors (TR), especially TRA. This isotype is particularly important for innate-like T cells, which typically express invariant TRA (iTRA). The presence of such cells in the rabbit remains an enigma. Rabbit NKT cells seem to be very rare, and lagomorphs lack MAIT cells. TRAV1, the variable gene expressed in the iTRA of these cells across most mammals, and MR1, the MH1-like receptor that present riboflavin derivatives to MAIT cells, are missing in rabbit. An alternative iTRA has been identified, that may be expressed by new innate-like T cells. To facilitate TRA repertoire analyses in rabbit, we report here a full description of TRA and TRD loci and a subgroup definition based on IMGT® classification. Rabbit TRA rearrangements follow the same temporal pattern that is observed in mouse and human. Rare transcripts expressing TRDV/TRDD/TRDJ rearrangements spliced to TRAC were detected. TRA and TRD genes have been made available in IMGT and IMGT/HighV-QUEST, allowing easy analysis of TRA/TRD RepSeq. © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Report this publication

Statistics

Seen <100 times