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T-cell factor 4 (Tcf7l2) maintains proliferative compartments in zebrafish intestine.

Authors
  • Muncan, Vanesa
  • Faro, Ana
  • Haramis, Anna-Pavlina G
  • Hurlstone, Adam F L
  • Wienholds, Erno
  • van Es, Johan
  • Korving, Jeroen
  • Begthel, Harry
  • Zivkovic, Danica
  • Clevers, Hans
Type
Published Article
Journal
EMBO reports
Publication Date
Oct 01, 2007
Volume
8
Issue
10
Pages
966–973
Identifiers
PMID: 17823612
Source
Medline
License
Unknown

Abstract

Previous studies have shown that Wnt signals, relayed through beta-catenin and T-cell factor 4 (Tcf4), are essential for the induction and maintenance of crypts in mice. We have now generated a tcf4 (tcf7l2) mutant zebrafish by reverse genetics. We first observe a phenotypic defect at 4 weeks post-fertilization (wpf), leading to death at about 6 wpf. The phenotype comprises a loss of proliferation at the base of the intestinal folds of the middle and distal parts of the intestine. The proximal intestine represents an independent compartment, as it expresses sox2 in the epithelium and barx1 in the surrounding mesenchyme, which are early stomach markers in higher vertebrates. Zebrafish are functionally stomach-less, but the proximal intestine might share its ontogeny with the mammalian stomach. Rare adult homozygous tcf4(-/-) 'escapers' show proliferation defects in the gut epithelium, but have no other obvious abnormalities. This study underscores the involvement of Tcf4 in maintaining proliferative self-renewal in the intestine throughout life.

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