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Systematic identification of key functional modules and genes in esophageal cancer

Authors
  • Wu, Rui1
  • Zhuang, Hao1
  • Mei, Yu-Kun2
  • Sun, Jin-Yu1
  • Dong, Tao1
  • Zhao, Li-Li1
  • Fan, Zhi-Ning1
  • Liu, Li1
  • 1 The First Affiliated Hospital with Nanjing Medical University, 300 Guangzhou Road, Nanjing, Jiangsu, 210029, China , Nanjing (China)
  • 2 Nanjing Medical University, 101 Longmian Avenue, Nanjing, Jiangsu, China , Nanjing (China)
Type
Published Article
Journal
Cancer Cell International
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Feb 25, 2021
Volume
21
Issue
1
Identifiers
DOI: 10.1186/s12935-021-01826-x
Source
Springer Nature
Keywords
License
Green

Abstract

BackgroundEsophageal cancer is associated with high incidence and mortality worldwide. Differential expression genes (DEGs) and weighted gene co-expression network analysis (WGCNA) are important methods to screen the core genes as bioinformatics methods.MethodsThe DEGs and WGCNA were combined to screen the hub genes, and pathway enrichment analyses were performed on the hub module in the WGCNA. The CCNB1 was identified as the hub gene based on the intersection between DEGs and the greenyellow module in WGCNA. Expression levels and prognostic values of CCNB1 were verified in UALCAN, GEPIA2, HCMDB, Kaplan–Meier plotter, and TIMER databases.ResultsWe identified 1,044 DEGs from dataset GSE20347, 1,904 from GSE29001, and 2,722 from GSE111044, and 32 modules were revealed by WGCNA. The greenyellow module was identified as the hub module in the WGCNA. CCNB1 gene was identified as the hub gene, which was upregulated in tumour tissues. Moreover, esophageal cancer patients with higher expression of CCNB1 showed a worse prognosis. However, CCNB1 ‘might not play an important role in immune cell infiltration.ConclusionsBased on DEGs and key modules related to esophageal cancer, CCNB1 was identified as the hub gene, which offered novel insights into the development and treatment of esophageal cancer.

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