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Systematic analysis of long non-coding RNA and mRNA expression changes in ApoE-deficient mice during atherosclerosis.

Authors
  • Lou, Xiaoqian1, 2
  • Ma, Xiaoyan3
  • Wang, Dawei4
  • Li, Xiangjun1
  • Sun, Bo1
  • Zhang, Tong1
  • Qin, Meng1
  • Ren, Liqun5
  • 1 Department of Experimental Pharmacology and Toxicology, School of Pharmacy, Jilin University, Changchun, 130021, Jilin, People's Republic of China. , (China)
  • 2 Department of Endocrinology, The First Hospital of Jilin University, Changchun, 130021, Jilin, People's Republic of China. , (China)
  • 3 Department of Cardiology, The Affiliated Hospital of Changchun University of Chinese Medicine, Changchun, 130021, Jilin, People's Republic of China. , (China)
  • 4 Department of Emergency, The First Hospital of Jilin University, Changchun, 130021, Jilin, People's Republic of China. , (China)
  • 5 Department of Experimental Pharmacology and Toxicology, School of Pharmacy, Jilin University, Changchun, 130021, Jilin, People's Republic of China. [email protected] , (China)
Type
Published Article
Journal
Molecular and Cellular Biochemistry
Publisher
Springer-Verlag
Publication Date
Dec 01, 2019
Volume
462
Issue
1-2
Pages
61–73
Identifiers
DOI: 10.1007/s11010-019-03610-y
PMID: 31446617
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Atherosclerosis plays an important role in the pathology of coronary heart disease, cerebrovascular disease, and systemic vascular disease. Long non-coding RNAs (lncRNAs) are involved in most biological processes and are deregulated in many human diseases. However, the expression alteration and precise role of lncRNAs during atherosclerosis are unknown. We report here the systematic profiling of lncRNAs and mRNAs in an ApoE-deficient (ApoE-/-) mouse model of atherosclerosis. Clariom D solutions for the mouse Affymetrix Gene Chip were employed to analyze the RNAs from control and ApoE-/- mice. The functions of the differentially expressed mRNAs and lncRNAs and the relationships of their expression with atherosclerosis were analyzed by gene ontology, co-expression network, pathway enrichment, and lncRNA target pathway network analyses. Quantitative real-time PCR (QRT-PCR) was used to determine the expression of mRNAs and lncRNAs. A total of 2212 differentially expressed lncRNAs were identified in ApoE-/- mice, including 1186 up-regulated and 1026 down-regulated lncRNAs (|FC| ≥ 1.1, p < 0.05). A total of 1190 differentially expressed mRNAs were found in the ApoE-/- mice with 384 up-regulated and 806 down-regulated (|FC| ≥ 1.1, p < 0.05). Bioinformatics analyses demonstrated extensive co-expression of lncRNAs and mRNAs and concomitant deregulation of multiple signaling pathways associated with the initiation and progression of atherosclerosis. The identified differentially expressed mRNAs and lncRNAs as well as the related signaling pathways may provide systematic information for understanding the pathogenesis and identifying biomarkers for the diagnosis, treatment, and prognosis of atherosclerosis.

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