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Synthetic promoter design for new microbial chassis.

Authors
  • Gilman, James1
  • Love, John2
  • 1 Biosciences, College of Life and Environmental Sciences, The University of Exeter, Stocker Road, Exeter EX4 4QD, U.K.
  • 2 Biosciences, College of Life and Environmental Sciences, The University of Exeter, Stocker Road, Exeter EX4 4QD, U.K. [email protected]
Type
Published Article
Journal
Biochemical Society Transactions
Publisher
Portland Press
Publication Date
Jun 15, 2016
Volume
44
Issue
3
Pages
731–737
Identifiers
DOI: 10.1042/BST20160042
PMID: 27284035
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The judicious choice of promoter to drive gene expression remains one of the most important considerations for synthetic biology applications. Constitutive promoter sequences isolated from nature are often used in laboratory settings or small-scale commercial production streams, but unconventional microbial chassis for new synthetic biology applications require well-characterized, robust and orthogonal promoters. This review provides an overview of the opportunities and challenges for synthetic promoter discovery and design, including molecular methodologies, such as saturation mutagenesis of flanking regions and mutagenesis by error-prone PCR, as well as the less familiar use of computational and statistical analyses for de novo promoter design. © 2016 The Author(s).

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